Objective：To investigate the expression of DNA dependent protein kinases catalytic subunit (DNApkcs) and Ku70 in gallbladder adenocarcinoma,peritumoral tissues,adenomatous polyp and chronic cholecystitis, and explore its clinicopathological significance.
Methods：EnVisionTM immunohistochemical method was used to detect the expression of DNApkcs and Ku70 in the routinely paraffin embedded sections of samples of gallbladder adenocarcinoma(n=108),peritumoral tissues(n=46), adenomatous polyp(n=15) and chronic cholecystitis(n=35).
Results：The positive rates of DNApkcs and Ku70 were significantly lower in gallbladder adenocarcinoma(50.0%，51.9%)than in peritumoral tissues(82.6%，82.6%)and chronic cholecystitis(91.4%，88.6%)(P＜0.05 or P＜0.01).The positive rates were significantly higher in the cases of well-differentiated adenocarcinoma, non-metastasis of lymph node and non-infilatrated regional tissues or organs than in those of low-differentiated adenocarcinoma,metastasis of lymph node and infilated regional tissues or organs (P＜0.05 or P＜0.01).A high consistence was found between DNApkcs and Ku70 expression in gallbladder adenocarcinoma.
Conclusions：The expression of DNApkcs and Ku70 might be important biological markers reflecting the carcinogenesis，progression，biological behaviors and prognosis of gallbladder adenocarcinoma.The cases with positive expression of DNApkcs and Ku70 might have better prognosis.