Objective:To study the expression of MAP3K10 in human pancreatic carcinoma (PC), and the relationship between MAP3K10 and human PC.
Methods:The expressions of MAP3K10 protein and mRNA in PC and adjacent non-tumorous tissues were detected by immunohistochemical staining, Western blot and quantitative Real-time PCR.
Results:(1)Immunohistochemical staining demonstrated that the number of positive staining cells in pancreatic carcinoma (100% strong positive) was notablely higher than that in matched adjacent non-tumorous tissues (only 25% weak posive) (P＜0.05);(2)Western blot proved that the expression of MAP3K10 protein in 91.7% of PC, which was higher than that in matched adjacent non-tumorous tissues; (3)qRT-PCR showed that the expression of MAP3K10 mRNA in PC was significantly higher than that in matched adjacent non-tumorous tissues（P＜0.01）.
Conclusions:MAP3K10 is abnormally activated in pancreatic carcinoma, might play an important role in the carcinogenesis and progress of pancreatic carcinoma, and is expected to become a new therapeutic target for pancreatic carcinoma.