Objective:To study the expression of MAP3K10 in human pancreatic carcinoma (PC), and the relationship between MAP3K10 and human PC. Methods:The expressions of MAP3K10 protein and mRNA in PC and adjacent non-tumorous tissues were detected by immunohistochemical staining, Western blot and quantitative Real-time PCR. Results:(1)Immunohistochemical staining demonstrated that the number of positive staining cells in pancreatic carcinoma (100% strong positive) was notablely higher than that in matched adjacent non-tumorous tissues (only 25% weak posive) (P<0.05);(2)Western blot proved that the expression of MAP3K10 protein in 91.7% of PC, which was higher than that in matched adjacent non-tumorous tissues; (3)qRT-PCR showed that the expression of MAP3K10 mRNA in PC was significantly higher than that in matched adjacent non-tumorous tissues(P<0.01). Conclusions:MAP3K10 is abnormally activated in pancreatic carcinoma, might play an important role in the carcinogenesis and progress of pancreatic carcinoma, and is expected to become a new therapeutic target for pancreatic carcinoma.
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LIN Fan, WANG Min, HONG Xiaoquan, LI Xu, WANG Xin, QIN Renyi. Expression and significance of MAP3K10 in pancreatic carcinoma [J]. Chin J Gen Surg,2011,20(3):257-260. DOI:10.7659/j. issn.1005-6947.2011.03.011