Objective：To explore the clinical value of strain ratio measurement with ultrasound elastography (UE) for diagnosis of breast neoplasm. Methods：A total of 109 breast masses in 103 patients for diagnosis in our hospital were randomly examined with 2-dimensional ultrasonography and elastosonography. The strain ratio of the lesion and nearby breast tissue was calculated, and the results were compared with final pathological findings. Results：There were 71 benign and 38 malignant lesions in the 109 breast masses. The strain ratios between benign lesion (2.26±1.39) and malignancy (6.95±4.08) were significantly different (P<0.05). The sensitivity, specificity and accuracy of 2-dimensional ultrasonography and strain ratio for breast cancer detection was 81.58%, 80.28%, 80.73% and 86.84%, 88.73%, 88.07% respectively (P>0.05). The rate of diagnostic sensitivity and accuracy was increased to 97.37% and 93.58% respectively (P<0.05) by combination of 2-dimensional ultrasonography and strain ratio measurement. Conclusions：Combined 2-dimensional ultrasonography with ultrasonic elastographic diagnostic method can elevate the coincidence rate of preoperative diagnosis of breast neoplasm.