Objective:To investigate the effect of absorbable hemostatic polysaccharide sponge on wound healing.
Methods:The open-wound animal models were established in Guangxi Bama minipigs. The porcine models were then randomized into group A (absorbable hemostatic polysaccharide sponge treatment group), group B (absorbable gelatin sponge treatment group) and group C (no treatment group). Wound healing responses were monitored at 1, 3, 5, 7, 14, 21 and 28 d after injury. The histopathological features and transforming growth factor-β 1 (TGF-β1) expression of the tissue from wound edge at each time point of each group were examined using HE and immunohistochemical staining and compared, respectively. Furthermore, the ultrastructure of the wound tissues at 7 d after injury was observed by transmission electron microscapy (TEM).
Results:The wound healing rate of group A was significantly higher than that of group B and C at the time points from 5 to 28 d after injury (P＜0.05). The positive expression rate of TGF-β1 in the specimens of group A was significantly higher than that of group B and C, especially at 7 d after injury (P＜0.05). The results of TEM demonstrated that the fibroblasts in wound tissues of group A were mainly synthetic and secretory type, while they were mainly proliferative type in wound tissues of group B and C.
Conclusions:Absorbable hemostatic polysaccharide sponge has accelerating effect on wound repair.