Objective：To screen out the aberrantly expressed miRNAs in the intestinal type gastric cancer.
Methods：The miRNA expression levels of 6 cases of intestinal type gastric cancer tissues and their adjacent non-tumour tissues were evaluated with miRCURYTM LNA microRNA array (v.14.0), and the average level  increased (or decreased) more than 2 folds and P value less than 0.01 were set as cutoff values. Part of the aberrantly expressed miRNAs revealed by microRNA array were selected and further analyzed in 29 cases of intestinal type gastric cancer tissues and their adjacent non-tumour tissues using quantitative real-time PCR (RT-qPCR), and the correlation between the two results was analyzed.
Results：As revealed by miRNA microarray analysis, 40 mRNA presented significant up-regulated expression, of which 24 genes had been found to be increased in gastric cancer tissue previuosly; 36 mRNA showed significant down-regulated expression, of which 19 mRNA had been reported to be abnormally decreased in gastric cancer previously. Six up-regulate miRNAs  and 5 down-regulated miRNAs in the microRNA array analysis were examined by RT-qPCR, and the results were consistent with the results of the microRNA array analysis. There was a significant positive correlation between the two methods (P<0.01).
Conclusions：Our study identifies a series of new miRNA aberrantly expressed in intestinal type gastric cancer and thereby provides a basis for further study.