Objective: To study the feasibility of sequential regional intra-arterial infusion (RAI) and drug combination schemes in treatment of severe acute pancreatitis (SAP). Methods: The clinical data of 45 patients with SAP undergoing early RAI treatment were retrospectively analyzed. Results: The drugs used in all cases included: ⑴ Disebrin saline (25 U/mL, used as the infusion vehicle for all drugs); ⑵ 5-FU (250 mg/12 h, used in the first week for 7 days); ⑶ Somatostatin or its analogue (0.4 mg sandostatin or 6 mg somatostatin, daily, for about 2 weeks); ⑷ Antibiotics (continuous infusion in 12 cases with secondary infection and prophylactic use for 2 weeks in 23 cases without secondary infection). The selectively used medications included: ⑴ Ulinastatin (10 000-20 000 U/12 h, in 5 cases complicated with MODS); ⑵ Prostaglandin E1 (10 μg/12 h, in 4 cases who had microcirculatory disturbance). Of the whole group, 2 cases died and 45 cases were cured. Conclusion: RAI is a feasible and effective modality for SAP patients, but care must be taken for drug selection and compatibility during infusion.