Objective: To investigate the microRNAs (miRNAs) expression profiles in human pancreatic cancer, so as to provide a basis for further exploring the role of miRNAs in the molecular mechanism that leads to the occurrence and development of pancreatic cancer. Methods: The differentially expressed miRNAs between the pancreatic cancer tissue and adjacent normal pancreatic tissue were analyzed by microRNA array, and then two of the differentially expressed miRNA were selected to perform quantitative real time PCR (qRT-PCR) assay, so as to validate the microRNA array results. Results: Compared with the normal pancreatic tissues, 15 miRNAs were more than 1.5-fold up-regulated, and 11 miRNAs were more than 1.5-fold down-regulated in pancreatic cancer tissues. The values of relative quantification (RQ) of miR-10b and miR-21 in pancreatic cancer tissues and their adjacent normal tissues from 8 cases detected by qRT-PCR were 0.0743±0.0222 vs. 0.0287±0.0129, and 0.3062±0.1117 vs. 0.0240±0.0137, respectively (both P<0.05), and these were consistent with the microRNA array results. Conclusion: The 26 differentially expressed miRNAs screened by microRNA array may contribute to the occurrence and development of pancreatic cancer, and their respective roles in this disease are worthy of further investigation.