Objective: To investigate the relevance of different anastomosis patterns to the incidence of pancreatic fistula after Roux-en-Y pancreaticojejunostomy (PJ). Methods: The clinical data of 47 patients with chronic pancreatitis and associated dilation of the main pancreatic duct undergoing Roux-en-Y PJ from Augest 1999 to June 2011 were retrospectively analyzed. According to the different anastomosis patterns (continuous or interrupted Prolene sutures), the patients were divided into two groups, and then the incidence of pancreatic fistula and clinical outcomes of the two groups after surgery were analyzed, respectively. Results: No pancreatic fistula occurred in the 25 cases with continuous sutures, while 4 of the 22 cases with interrupted sutures developed pancreatic fistula, and the difference between the two group had statistical significance (P<0.05). The postoperative follow-up ranged from 3 to 5 years, and the abdominal pain was completely relieved in 46 cases. Conclusion: Interrupted suture is prone to developing pancreatic fistula after Roux-en-Y PJ. However, the continuous Prolene suture technique may reduce the incidence of pancreatic fistula and this procedure is also simple and easy to perform.