Objective: To investigate the therapeutic effect of in vitro amplified bone marrow c-Kit+Lin? (CD117) cells transplantation on hepatic fibrosis in mice. Methods: The bone marrow c-kit+Lin? cells of mice were isolated and purified by using magnetic-activated cell sorting (MACS), and then the cells were cultured and expanded in the serum-free medium supplemented with hepatocyte growth factor (HGF), stem cell factor (SCF), FLt-3 ligand (FL), thrombopoietin (TPO) and leukemia inhibitory factor (LIF). The models of alcohol induced hepatic fibrosis were created in mice of the same strain, and the model mice were treated with CD117 cells (transplantation group) or buffer solution of the same volume (model group) by tail vein injection respectively, and the normal mice were used as control (normal group). Thirty days later, the pathological changes of liver and the parameters concerning liver function and hepatic fibrosis of each group were assessed. Results: The CD117 cells were effectively expanded in the serum-free medium supplemented with the above mentioned factors. No pathological alteration was noted in the liver of normal mice, while the liver of model group presented remarkable hepatocyte degeneration and fibrotic changes, and these alterations were obviously alleviated in transplantation group compared with model group. In both model and transplantation groups, the serum levels of alanine transaminase (ALT), aspartate transaminase (AST), hyaluronic acid (HA) and laminin (LN) increased, and albumin (ALB) decreased with varying degrees compared with normal group, but the alterations of the above parameters in transplantation group were all milder than those in model group, and all differences had statistical significances (all P<0.05). Conclusion: CD117 cells can be effectively expanded in vitro by the proper combination of cytokins (HGF, SCF, FL, TPO and LIF). CD117 cells transplantation has therapeutic effect on hepatic fibrosis in mice.