Objective: To compare the clinical efficacies between two methods of selective hepatic vascular exclusion in laparoscopic hepatectomy (LH). Methods: The clinical data of 28 patients undergoing LH from May 2005 to June 2011 were retrospectively analyzed. According to the procedures of selective hepatic vascular exclusion, the patients were divided into group A (exclusion through Glisson's capsule, n=14) and group B (exclusion through separated blood vessels after Glisson's capsule dissection, n=14). The perioperative parameters (operative time, intraoperative blood loss and blood transfusion volume), postoperative indexes (routine blood tests, liver function parameters, postoperative complications and hospital stay) and clinical outcomes (recurrence and survival rate) between the two groups were compared. Results: There were no significant differences in the operative time, bleeding, and transfusion volume between the two groups (all P>0.05). No significant differences were noted in parameters of liver function and routine blood tests on each postoperative time point, as well as in length of postoperative hospitalization and complications between the two groups (all P>0.05). The follow-up results showed that there were no significant differences in 1- and 2-year recurrent rate as well as 2- and 3-year survival rate between the two groups (all P>0.05). Conclusion: The two methods of selective vascular exclusion have no significant difference for LH, and the selection is on the surgeon's practice profile and surgical procedures.