Objective: To investigate the expression level of tumor suppressor gene WTX in colon cancer tissues and its significance. Methods: The specimens of cancerous tissues along with their adjacent and distant tissues from 50 patients with pathologically confirmed colon cancer were collected. The gene and protein expression levels of WTX in each tissue specimen were detected by using Real-time fluorescence quantitative PCR (qPCR) and Western blot analysis, respectively. Results: Relative to the value of the distant normal tissues (1.00±0.00), the WTX gene expression values in the tumor tissues and adjacent tissues were 0.36±0.17 and 0.91±0.34 respectively, and there were statistical differences among them (all P<0.05). The relative expression levels of WTX protein in the tumor tissues, adjacent and remote tissues were 0.27±0.11, 0.64±0.23 and 0.72±0.19 respectively, and all the differences among them had statistical significance (all P<0.05). Conclusion: The decreased expression of tumor suppressor gene WTX may be critically associated with the occurrence and development of colon cancer.