Objective: To investigate the hepatoprotective effect of hypothermic infusion of self-prepared hepatic protective solution during portal vein occlusion (PVO) in rats with obstructive jaundice. Methods: Seventy-two rats underwent common bile duct ligation, and one week later were equally randomized into control group (recanalization of bile duct and only portal vein isolation), portal vein obstruction (PVO) group (recanalization of bile duct and PVO for 1.5 h), lactated Ringer’s solution infusion group (recanalization of bile duct and portal vein infusion with 4 ℃ lactated Ringer’s solution during PVO) and self-prepared hepatic protective solution infusion group (recanalization of bile duct and portal vein infusion with 4 ℃ self-prepared hepatic protective solution during PVO). The specimens of rats’ livers from each group were harvested at 0, 6 and 24 h respectively after surgery, and then the expressions of bcl-2, Bax and caspase-3 protein in liver tissues were detected by Western blot analysis, and the liver cell apoptosis rates were measured by TUNEL assay. Results: Compared with control group, the bcl-2 expression in the liver tissues of PVO group increased slightly at each time point after surgery, but all the differences had no statistical significance (all P>0.05), while the bcl-2 expressions in the liver tissues of the two solution infusion groups were significantly increased at 6 and 24 h after surgery (all P<0.05), which were more evident in self-prepared hepatic protective solution infusion group (all P<0.05); the expressions of Bax and caspase-3 protein in the liver tissues as well as liver cell apoptosis rates of PVO group significantly increased at 6 and 24 h after surgery (all P<0.05), and the two infusion solutions significantly inhibited the elevation of Bax and caspase-3 protein expression and reduced the liver cell apoptosis rate (all P<0.05), and these effects were more pronounced in self-prepared hepatic protective solution infusion group (all P<0.05). Conclusion: Self-prepared hepatic protective solution has hepatoprotective effect during PVO in rats with obstructive jaundice, and the mechanism is related to the inhibition of liver cell apoptosis of mitochondrial pathway.