Objective: To analyze Hepatitis B virus (HBV) genotypes and drug resistance mutations among people in Zhangjiakou. Methods: The genotypes and drug resistant mutation of HBV in 98 infected cases of Han nationality in Zhangjiakou were detected by nested quantitative real-time PCR and real-time fluorescent PCR. Results: The main genotypes among the 98 cases in Zhangjiakou were genotype B (29.59%), C (30.61%) and B+C (39.80%). The total mutation rate of YMDD was 58.16% (57/98), among which, YVDD mutation accounted for 61.40% (35/57), and YIDD mutation was 38.60% (22/57). The YMDD mutation rate in genotype B was 21.43% (21/98), C was 33.67% (33/98) and B+C was 44.90% (44/98) respectively, and the difference among them was statistically significant (P<0.05). Conclusion: The main HBV genotypes of Han nationality in Zhangjiakou are genotypes B, C and B+C, and the genotype B+C has the highest YMDD mutation rate after Lamivudine treatment.