Pancreatic cancer is one of the most malignant diseases with the poorest prognosis, so the investigation into its pathogenesis is of great clinical importance. MicroRNAs (miRNAs) participate in various biological processes via controlling gene expression, and the aberrant expression of miRNAs is closely associated with initiation and progression of tumors as well as correlated with the prognosis. In this paper, the authors present the recent advances regarding the relations of miRNAs with the diagnosis, treatment and prognosis of pancreatic cancer.