Objective: To investigate the standard operation procedures (SOP) to establish a breast cancer tissue bank, which can provide a basis for establishment of an integrated and standardized breast cancer tissue bank with information-based management. Methods: The fresh tumor tissues along with their adjacent tissues and distal normal tissues were obtained from breast cancer patients during surgery, and blood samples were also drawn from some of these patients to extract the serum, plasma and lymphocytes. All the tissue samples were cryopreserved. A set of information management principles was adopted to manage the tissue specimens. Results: A total of 520 fresh tissue and blood samples from 22 breast cancer patients were collected, of which, 2 were intraductal carcinoma, 3 were lobular carcinoma in situ, 10 were invasive ductal carcinoma, and 7 were invasive lobular carcinoma. Complete sets of tissue samples were obtained from 19 cases. The average handling time for sample collection and conservation was (19.38±10.16) min. The quality detection results derived from 20 randomly selected tissues showed that 90% of the tissue samples met the requirements for use. Conclusion: The establishment of an integrated breast cancer tissue bank with standardized procedures for collection of biospecimens and information-based management may provide material and information resource for basic and clinical research of breast cancer.