Objective: To observe the influence of hemoperfusion plus hemodialysis on CD8+CD28– T cells and systemic inflammatory state in patients with severe acute pancreatitis (SAP). Methods: Fifty-two patients who were admitted from February to September 2013 with confirmed SAP were selected, and equally randomized into observational group and control group. Patients in control group received routine treatment, while those in observational group underwent hemoperfusion plus hemodialysis in addition to receiving routine treatment. The serum levels of CD8+CD28– T cells, inflammatory factors and amylase after 24-h treatment, and the recovery patterns of clinical symptoms between the two groups were compared. Results: There was no statistical difference in the laboratory parameters between the two groups before treatment (all P>0.05). After 24-h treatment, the serum levels of CD8+CD28– T cells and anti-inflammatory cytokines were significantly increased, while the levels of pro-inflammatory cytokines and amylase were significantly decreased in both groups, but all the degrees of improvement in all the parameters in observational group were greater than those in control group (all P<0.05). The time to disappearance of clinical symptoms and bowel function recovery, and the length of hospital stay in observational group were all shorter than those in control group (all P<0.05), while no statistical difference was found in the time for body temperature recovery between the two groups (P>0.05). Conclusion: Application of hemoperfusion plus hemodialysis can effectively heighten the levels of CD8+CD28– T cells and inhibit the systemic inflammatory response in SAP patients, and thereby accelerate the recovery of the patients.