Objective: To investigate the p28GANK expression in pancreatic ductal carcinoma and its clinical significance. Methods: The p28GANK expression in pancreatic ductal cancer and matched adjacent non-cancerous tissues from 47 patients were determined by immunohistochemical staining. The relations of p28GANK expression in pancreatic ductal cancer with the clinicopathologic factors and postoperative survival of the patients were analyzed. Results: The p28GANK expression in pancreatic ductal cancer tissues was significantly higher than that in their adjacent non-cancerous tissues (P<0.01). The high p28GANK expression in tumor tissue was significantly associated with advanced TNM stage, low differentiation, and metastases(all P<0.05), but was irrelevant to age and gender of the patients (both P>0.05).The 3-year postoperative survival rate in patients with p28GANK positive expression was significantly lower than that in those with p28GANK negative expression (P=0.01). Conclusion: p28GANK expression is up-regulated in pancreatic ductal carcinoma, and high p28GANK expression is closely related to the progress of malignancy stage.