Objective: To evaluate the application value of fast-track surgery (FTS) concept in minimally invasive gallbladder-preserving operation. Methods: Sixty patients with gallstone or polypoid lesions of the gallbladder undergoing minimally invasive gallbladder-preserving operation from January 2010 to December 2013 were randomly designated to observational group and control group, with 30 cases in each group. Patients in observational group received FTS perioperative care, while those in control group were given the traditional perioperative management. The length of postoperative hospital stay, duration of postoperative transfusion, hospitalization expenses, alteration in postoperative C-reactive protein (CRP) levels and incidence of postoperative complications as well as the time and degree of gallbladder function improvement were compared between the two groups of patients. Results: In observational group compared with control group, the length of postoperative hospital stay, duration of postoperative intravenous fluid infusion and hospitalization cost were reduced, the increasing degree of postoperative CRP level was lessened with a shortened duration, the time to gallbladder function recovery was decreased and the degree of gallbladder function recovery was increased. All differences had statistical significance (all P<0.05). There was no statistical difference in incidence of postoperative complications between the two groups (P>0.05). Conclusion: In minimally invasive gallbladder-preserving therapy, using FTS conception with enhanced preoperative management and reduced stress responses to medical procedures can help accelerate gallbladder function recovery, shorten the length of hospital stay, and thereby facilitate the recovery of patients.