Objective: To investigate the influence of WTX gene on biological behaviors of human gastric cancer SGC-7901 cells. Methods: The recombinant plasmids bearing WTX gene or empty plasmid vectors were transfected into SGC-7901 cells by using Attractene reagent, and the untreated SGC-7901 cells were used as blank control. The EGFP-tagged transfection efficiency at different transfection times was determined, the WTX mRNA expression was measured by RT-PCR method, the cell proliferation was detected by CCK-8 assay, and the apoptosis and cell cycle were analyzed by flow cytometry. Results: The strongest expression of eGFP presented at 48 h after WTX gene transfection, when the transfection efficiency reached (33.10±4.16) %. In SGC-7901 cells of WTX transfection group compared with either blank control group or empty vector group, the WTX mRNA expression was increased significantly, proliferative ability was decreased significantly, and the number of S-phase cells was increased while the number of G1- and G2/M-phase cells was decreased significantly (all P<0.05). There was no significant apoptosis in any of the groups of cells. Conclusion: WTX gene can inhibit proliferation through inducing S-phase arrest in SGC-7901 cells, but has no influence on cell apoptosis.