Background and Aims Sentinel lymph node biopsy (SLNB) has become a standard treatment method for early breast cancer patients with negative axillary lymph nodes. The success or not of SLNB is closely related to the choice of tracer. Radioisotope and methylene blue are the two most widely used tracers in clinical practice. However, because the application of SLNB in China is lagged behind as compared to other countries, the current investigation showed that only 14.55% hospitals use the double-tracer technique of radioisotope plus methylene blue dye, with 62.73% hospitals still mainly use the single tracer method of methylene blue dye. There are few summary studies on the combined use of tracers. This study was conducted to evaluate the application value of radioisotope plus methylene blue dye method in SLNB of breast cancer in China by Meta-analysis.Methods The publicly published studies comparing using radioisotope plus methylene blue dye and single methylene blue dye method in SLNB performed by Chinese medical institutes were searched in Wangfang Data, CNKI, VIP database, Pubmed and Cochrane Library from the inception to December 2020. The literature was screened strictly according to the inclusion and exclusion criteria, and the data of the included studies were extracted. Meta-analysis was carried out by Revman 5.4 software, and the differences in the detection rate and detection number of sentinel lymph nodes (SLNs), as well as the false negative rate, sensitivity and accuracy rate between the two methods were compared. The publication bias was evaluated by funnel plot.Results A total of 18 studies were finally included, and all of them were domestic studies. Meta-analysis results showed that the identification rate of SLNs (OR=5.81, 95% CI=4.04-8.37, P<0.000 01), sensitivity (OR=3.35, 95% CI=2.17-5.17, P<0.000 01) and accuracy rate (OR=3.45, 95% CI=2.20-5.41, P<0.000 01) were significantly increased and the false negative rate (OR=0.26，95% CI=0.17-0.39, P<0.000 01) was significantly decreased by using method of radioisotope plus methylene blue dye compared with the single methylene blue tracer method. The detection rates of SLNs reported in the 18 articles were used to assess publication bias, and the funnel plot showed that a low possibility of publication bias.Conclusion Radioisotope combined with methylene blue dye method has good application value in SLNB of breast cancer in China. It is expected to be widely used in majority of hospitals in China.