Objective: To screen the differentially expressed genes in pancreatic carcinoma versus paired normal adjacent tissue by gene expression profile chip. Methods: The surgical specimens of pancreatic ductal cell carcinoma and adjacent normal pancreatic tissue from 10 patients from January 2014 to June 2016 in the Third Affiliated Hospital of Xinjiang Medical University were collected, and differentially expressed genes between them were screened by an Affymetrix gene expression profile chip containing 49 395 gene probes after total RNA extraction by TRIzol method, and then, GO and pathway analyses were performed. Finally, some differentially expressed genes were verified by real-time PCR. Results: The total RNA of samples passed the test, gene chip assessment yielded high-quality information and results of the test were reliable and highly repeatable; 38 079 genes were examined after denoising process of the chips, involving 512 differentially expressed genes that included 419 up-regulated genes and 93 down-regulated genes; there were 287 differentially expressed genes encoding proteins that were related to the three GO ontologies (biological process, molecular function and cellular component), and 29 signaling pathways were associated with significant differential gene expressions, involving 126 genes. The expressions of differentially expressed genes CPB1, CELA3B, CPA1, POSTN, PLA2G1B, CTRC and SPINK1 determined by real-time PCR were consistent with the results of gene chip analysis. Conclusion: There are a large number of differentially expressed genes between pancreatic ductal cell carcinoma and normal adjacent pancreatic tissue. These genes are mainly associated with biological processes, molecular functions and cellular components, and also involved in the regulation of multiple cellular signaling pathways.