Objective: To investigate the feasibility and effectiveness of nursing intervention based on multidisciplinary collaboration and integration in patients undergoing per-oral cholangiopancreatoscopy (PCPS) during the period of diagnosis and treatment. Methods: Ninety-six patients with pancreatobiliary duct diseases requiring PCPS treatment were randomly allocated by the order of admission to observation group (49 cases) and control group (47 cases). During the period of diagnosis and treatment, the patients in control group received conventional nursing intervention, while those in observation group received nursing intervention based on multidisciplinary collaboration and integration, which mainly included the informed consent, discussion before diagnosis and treatment, risk assessment, psychological preparation of patient, PCPS specific preparation, cooperation between medical and nursing care, nurse-patient cooperation and the intra- and postoperative supervision. Using the State-Trait Anxiety Inventory (STAI), Frankl Scale and self-designed questionnaires, the degree of anxiety, medical compliance, comfort level and satisfaction rate of the patients of the two groups were assessed, and the systolic blood pressure, heart rate and complications of the patients were also recorded. Results: Observation group was significantly superior to control group in terms of degree of anxiety, changes in systolic blood pressure and heart rate, patients’ medical compliance and comfort levels (all P<0.05). Meanwhile, the satisfaction rate was significantly increased after intervention and the incidence of treatment-associated complications was significantly reduced (all P<0.05). Conclusion: Through nursing intervention based on multidisciplinary collaboration and integration during the period of diagnosis and treatment, the stress discomforts such as anxiety and depression can be alleviated, the changes in blood pressure and heart rate can be reduced, the medical compliance, comfort level and satisfaction rate can be improved, and the incidence of complications can be decreased in patients undergoing PCPS.