Pancreatic cancer stem cells play an important role in the occurrence, development and metastasis as well as the therapeutic resistance and recurrence of pancreatic cancer, but the regulatory mechanisms involved are still unclear. In-depth study of the role of specific microRNA in the regulation of pancreatic cancer stem cells is of great significance for elucidating the key mechanisms of pathogenesis of pancreatic cancer, and is expected to provide a new target for the diagnosis and treatment of pancreatic cancer.