Germinal center kinases (GCK) are a type of serine/threonine protein kinase characterized by having a conserved serine/threonine protein-kinase domain, and together with p21-activated protein-kinases (PAK), they belong to the sterile twenty (Ste20)-like kinase family, which are involved in a wide variety of physiological processes, and some of them can cause stress responses to external stimuli, such as cell proliferation, apoptosis, skeleton rearrangement. Stk25 as an important· member of the subgroup III of the GCK family (GCKIII), in addition to participating in the above-mentioned physiological processes, simultaneously exerts important functions in energy metabolism and the occurrence and development of tumors. Here, the authors address the structural characteristics, biological effects, energy metabolism regulation and anti-tumor effects of STK25, so as to provide a reference for its translation into clinical treatment of tumors.