Liver needle biopsy has been used for a long time as a diagnostic measure for liver disease in several clinical departments such as liver surgery, liver transplantation, infectious disease, and digestive disease. Because it is usually performed by selecting a safe puncture route under the guidance of B-ultrasound or CT, it is safe and its incidence of postoperative massive hemorrhage or bile leakage is not high in most cases. However, there have been clinical reports of death due to liver needle biopsy, and this raised a great concern by clinicians. At present, there are no standards, guidelines or expert consensus on liver needle biopsy in China. Therefore, this expert consensus statement was developed through the organization by the Department of Medical Affairs and Department of Liver Surgery of Xiangya Hospital, Central South University in collaboration with the experts in own hospital from the Department of Hepatopathy, Ultrasound, Interventional Radiology and Pathology, so as to provide a reference for  performing liver needle biopsy in clinical practice.