Abstract:Objective
To investigate the expression of oncogenes and tumor suppressor genes in hepatitis B virus (HBV) related hepatocellular carcinoma (HCC).
Methods mRNA was extracted from cancerous and paracancerous tissues of 22 patients with HBVrelated HCC and synthesized into cDNA. The cDNA labeled with 33pdATP was hybridized for cDNA microarray each consisting of 3170 genes or expressed sequence tags (EST). The differential expression genes were searched in gene data base and verified using RTPCR.
Results The differential expression of 1369 genes or EST was identified including 121 genes or EST altered 2 times or more in cancerous tissues compared with paracancerous tissues. Compared with paracancerous tissues, 88 showed overexpression and 33 genes were downregulated. The positive expression of transmembrane 4 superfamily member 1 (TM4SF1) in cancerous tissues was 86.4% and could not be detected in paracancerous tissues （P＜0.001）. The positive expression of suppression of tumorigenicity 14 (ST14) in paracancerous tissues of HBVrelated HCC patients was 72.8% and could not be detected in cancerous tissues （P＜0.01）.
Conclusions The positive expression of TM4SF1 in cancerous tissues，and ST14 in paracancerous tissues of HCC were related to the development of HBVrelated HCC.