Abstract:Objective
To investigate the inhibitory effect of vitamin E succinate(VES) combined with chemotherapeutic drugs on the proliferation of human breast cancer cells. Methods Bcap37 human breast cancer cells were treated with VES combined with chemotherapeutic drugs for 24h and 36h. The concentrations of VES were 10μg/mL and 20μg/mL and those of 5florouracil, mitomycin and cyclophosphamide were 16.9μg/mL and 33.8μg/mL, 1μg/mL and 3.3μg/mL and 100μg/mL and 300μg/mL respectively. The inhibitory effect was measured with MTT method and the cell cycle and cell surface Fas expression were analyzed with flow cytometry assay. Results The combination of VES with chemotherapeutic drugs had a significant inhibitory effect on the growth of Bcap37 human breast cancer cells. Flow cytometry assay of cell cycle showed that the natural apoptptic rate of Bcap37 cells was 0.7%;after treatment with VES 20μg/mL,the apoptotic rate was 19.2%;after treatment with 5Fu,mitomycin and cyclophosphamide the apoptotic rates were 16.2%,16.7% and 12.3%,respectively;after the combined use of VES and the 3 chemotherapeutic drugs,the apoptotic rates were 40.3%,44.8%,39.6%,respectively.Fas expression in cancer cells increased after the coadministration of VES and chemotherapy drugs.
Conclusions VES combined with chemotherapeutic drugs had significant inhibitory effect on the growth of Bcap37 human breast cancer cells. The mechanism may be related to Fas upregulation on the surface of cancer cells.