Abstract:Objective
To investigate the effect of ischemic preconditioning (IPC) on pancreas transplantation(PT) in rats.
Methods Steptozozininduced diabetic SD rats were randomly assigned to 2 groups: group I/R(ischemia/reperfusion), consisted of 30 diabetic rats which received PT; group IPC, consisted of 30 diabetic rats which received pancreas transplantation and IPC. Six rats in each group were randomly sacrificed at 2 days before PT, and 3 days and 7days after PT, to detect the level of blood sugar and amylase,and pancreatic sections were stained with HE simultaneously; 6 rats were used to observe various metabolic indexes, and other 6 rats were used to observe the rat survival rate. Results The rats of group IPC had a higher 1 month survival rate than group I/R (5/6 vs 3/6, P<0.01). The blood sugar and amylase activity,food intake, water intake and urine volume in group IPC were lower than group I/R at each time period after transplantation(P<0.01, P<0.01, P<0.05 ,P<0.05, P<0.01,respectively). The degree of injury of pancreas graft in group I/R was more serious than that in group IPC. Conclusions IPC can improve the survival rate, reduce amylase activity, alleviate the degree of injury of pancreas graft, and protect the pancreas graft after PT in rats.