Abstract:Objective
To study the protective effects of adenosine（ADO） on reperfusion injury following pancreaticoduodenal transplantation in rats.
Methods The homologous male Wistar rat model of heterotopic total pancreaticoduodenal transplantation was made. The rats received IV injection of either ADO,8PT(1,3dimethyl 8phenylxanthine) or ADO+8PT at 5 min before or 5 min after operation,and then were divided into 6 groups:(1)blank control(C group);(2)transplant control group;(3)transplant +ADO group(ADO);(4)transplant +8PT group(8PT);(5)transplant+ADO+8PT group(ADO+8PT),and (6) transplant+［α32P］ ADO(radiationvisible group).AT 24h after transplantation,the pancreatic tissue concentration of adenosine triphosphate（ATP） and total adenine nucleotides (TAN) were determined, blood sugar (BG) and serum concentration of amylase and lipase were examined, the activity of myeloperoxidase (MPO) in pancreatic graft tissue was measured, and histological observation was made. ［α32P］ADO was used to radiologically identify the content of ADO in pancreatic tissue.
Results Pancreatic tissue concentration of ATP and TAN［（5.72±0.47）μmol/g, （8.71±0.91）μmol/g］in ADO group> transplant control group［（3.39±0.58）μmol/g, （5.01±0.78）μmol/g］>(ADO+8PT)group［（2.92±0.24）μmol/g, （4.84±0.41）μmol/g］>8PT group［（2.05±0.66）μmol/g, （3.98±0.41）μmol/g］ 24h after transplantation（P<0.05）.The value of BG and serum lipase and MPO in ADO group< transplant control group < (ADO+8PT)group < 8PT group(P<0.05). Lightmicroscopic studies showed that histomorphological changes of pancreas in experimental group were muchless than that in other groups.Radioactive ［α32P］ADO was found that the radiactive meteria can enter the pancreatic acinar cells.〖WTHZ〗Conclusions〓〖WTBZ〗Exogenous ADO can increase the amount of ATP and TAN in pancreatic graft tissue,while 8PT reduces it. ADO has protective effects on the ischemia/reperfusion injury of pancreaticoduodenal graft.