Abstract:Objective：To investigate the expression of 5-LOX in pancreatic cancer tissue and the relationship between expression of 5-LOX and COX-2. Methods：The expression of 5-LOXmRNA and COX-2mRNA in 35 fresh pancreatic cancer tissue samples were detected by semi-quantitive reverse transcriptase-polymerase chain reaction method. Results：Expression of 5-LOX mRNA and COX-2mRNA in pancreatic cancer tissue was 74.3%, 80%, respectively. Expressions of 5-LOX mRNA and COX-2 mRNA were not coordinated(P>0.05). There was correlation between the expression of 5-LOX and TNM stages. The expression of 5-LOX in stages III~IV of pancreatic cancer was markedly higher than that in stages I~II of pancreatic cancer（P<0.05）. Conclusions：Expression of 5-LOX and COX-2 in pancreatic cancer tissue was markedly elevated, and correlated with clinical stage. Expressions of 5-LOX and COX-2 in pancreatic cancer were not coordinated.