Abstract:Objective:To observe the effects and mechanism of antisense HIF-1α gene on implanted human primary hepatic carcinoma of nude mouse．Methods:Tumors were established by subcutaneous injection of 3×106 tumor cells，named SMMC-7721，into the back of nude mice．Once the tumor grew to 0.4cm in diameter, the mice were divided randomly into three groups with injected respectively with NS、empty plamid PcDNA3、and antisense HIF-1α plasmid．Half group of the nude mice were used to observe the tumor growth curve，and the other half were to obtain the tumor samples to be used in the examinations of the expression of angiostatin、HIF-1α, VEGF, MVD and cell apoptosis．Results:Tumors grew rapidly in the control group than that in the antisense HIF-1α group. In antisense HIF-1α gene therapy resulted in low expression of HIF-1α, MVD and VEGF than those in 2 control groups(P<0.05~0.01); but in HIF-1α group. AI was higher than that in the control group（P＜0.01）.Conclusions:Antisense HIF-1α gene therapy is a promising approach to treat human liver cancer by suppression of tumor growth and tumor angiogenesis and by induction of cell apoptosis．