Abstract:Objective：To compare the postoperative changes in immune function of patients undergoing robot-assisted laparoscopic cholecystectomy(RLC) and conventional laparoscopic cholecystectomy (CLC). Methods：The clinical data of 52 cases of gallbladder stones and gallbladder polyps treated with RLC(23 cases), and with LC(29 cases) was compared. Blood samples from both groups were taken to measure immune markers (T cells, IgA, IgM, IgG, C3, C4) before operation and in the first postoperative morning. Results：The postoperative levels of CD4/CD8 and IgM decreased from 1.56±0.67, 1.42 ±0.58 to 1.30±0.53, 1.26±0.46 respectively after RLC（all P<0.05）. C3 and C4 after CLC decreased from 1.48±0.34, 0.25±0.09 to 1.38±0.28, 0.22±0.07 respectively(all P<0.05). But the levels of immune markers before and after operation were not significantly different between RLC and CLC groups（P>0.05）. Conclusions：RLC and CLC showed no significant postoperative effect on cellular immune function, but both had some effect on humoral immune function.