Abstract:Objective：To explore the effects of administration of chondritin-sulfate(CS) on acute necrotizing pancreatitis(ANP) and its associated tissue oxidation stress injury.Methods：Male Wistar rats(n=90) were divided randomly into three groups：group A, ANP group; group B, ANP rats received chondroitin-sulfate therapy; group C, control group．Rats in three groups were killed at 30min,1h,3h,6h,and 12h after operation, respectively．The levels of pancreatic indexes (pancreatic wet/body weight),malonyl dialdehyde (MAD)、total superoxide dismutase (SOD),glutathione (GSH)、ATP and serum amylase (SAM) were measured．Changes of adhesion junctions were examined by using a electron microscopy．The cellular distribution and changes of localizetion of E-cadherin was observed by confocal fluorescence microscopy; an adhesion junction protein expression was studied by western-blot method．Results：Compared with group B, Levels of GSH、SOD and ATP in group A decreased markely （P<0.01）,however MDA increased significantly (P<0.01).In group A，compared with B and C group, it demostrated a rapid and sustained increase in levels of pancreatic indexes(P<0.01); damages of adhesion junctions E-cadherin was localized at the cytoplasm, the level of E-cadherin protein was markedly decreased(P<0.01)．Conclusions：In the early stage of experimental ANP, in pancreat tissue, the CSH, SOD and ATP decredsed significantly,resulting in severe damage of adhesion junction，which was responsible for the formation of pancreatic edema．Treatment with CS can protect adhesion junction by increasing E-cadherin protein concentration, and thus relieve pancreatic edema．