Abstract:Objective：To study the effect of cryopresevation of vitrified microencapsul in preserving the rat hepatocytes, and to select the best cryopreservation condition of hepatocytes. Methods：33%,37%,40% ethyl hydroxide was respectively used as the cryopreserving agent of hepatocytes, and the effect of different speeds of cryopreservation on hepatocytes conservation was tested. Results：The best cryopreseration agent of hepatocytes was 40% ethyl hydroxide; the effect of different speeds of cryopreservation on hepatocytes preservation was the same. The P450 activation and urea synthesis function of cryopreserved hepatocytes were as same as before and after cryopresevation. Conclusions：Vitrification can presere the microencapsuled rats hepatocytes effectively.