Abstract:Objective ：To investigate the protective effect of extract of Ginkgo biloba (EGb) on intestinal mucosal barrier after rats pancreas transplantation(PT), and analyze the possible mechanism. Methods ：In the control group, 12 normal rats received sham operation, and 24 steptozozin-induced diabetic SD rats were randomly assigned to 2 groups: Group PT consisted of 12 diabetic rats which received pancreas transplantation, group EGb consisted of 12 diabetic rats that received EGb（1.5 mg/kg） injected intravenously 1 day and 30min respectively before pancreas transplantation. The intestinal permeability, absorptional function and morphology, the TNF-α, NO, SOD and amylase activity in serum, and MDA, SOD and MPO in interstitial tissue were monitored 5 days after PT. Simultaneously, bacteria translocation in the superior mesenteric vein, mesenteric lymph node, liver and spleen was observed. Results ：(1)The mean TNF-α level( P <0.01) and amylase activity in serum( P <0.01), the mean intestinal permeability ( P <0.01), the mean bacteria translocation rate( P <0.01), the mean degree of MDA level and MPO activity( P <0.05) in intestinal mucous, and intestinal mucosal injury of group EGb were lower than those in Group PT. (2)The mean NO level ( P <0.01) and SOD activity ( P <0.01) in serum and the intestinal absorption function of group EGb were higher than those in Group PT. Conclusions ：EGb can protect the intestinal mucosal barrier of pancreas transplant recipient, and decrease the rate of bacteria translocation. The mechanism may be related to antioxidation and elimination of free radicals, decrease synthesis of TNF-α, decrease of adhesion and aggregation of PMNs, and increase production of endogenous NO.