Abstract:Objective:To identify the anticoagulation effect of pcDNA3.1 transfected exogenous human throbomodulin molecule (hTM) on umbilical vein endothelial cells (HUVECs). Methods:The pcDNA3.1/hTM plasmid was transduced into HUVECs by cationic liposome. Expression of hTMmRNA and hTM protein was detected by RT-PCR and immunohistochemistry,respectively. Results:The expression of hTMmRNA in HUVECs transduced with pcDNA3.1/hTM was 1.7 times higher than cells without transfected PcDNA3.1/hTM. Immunogistochemical examination showed about 10% cell tranfected with PcDNA3.1/hTM and high-level hTM protein was detected on positive cells. Conclusions:The pcDNA3.1/hTM plasmid could be transfected into endothelial cells by cationic liposome and the exogenous hTM protein had complete anticoagulation biological activity.
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QIAO Zheng-rong, YU Shen-lin, DAI Yi, GUO Ying.A study of endothelial cells modified by pcDNA3.1/hTM [J]. Chin J Gen Surg,2006,15(12):11-919. DOI:10.7659/j. issn.1005-6947.2006.12.011