Abstract:Objective：To study the inhibit effect of survivin short hairpin RNA on the survivin mRNA and protein expression of gallbladder carcinoma cells. Methods：Human gallbladder carcinoma cells(GBC-SD) were transfected with recombinant plasmid. RT-PCR and Western blot were used to analyze the changes in expression levels of survivin mRNA and protein. Results：The size of the PCR product was 350bp. DNA sequencing showed that the sequence of recombinant vector pshRNA-survivin was successfully constructed and suppressed the expression of (GBC-SD) survivin mRNA. Conclusions：The recombinant plasmid constructed can inhibit the expression of survivin mRNA in transfected cells. This provides a new method and material for the biological therapy of cancer.