Abstract:Objective:To study the activation effects to cytotoxic T lymphocytes by gastric cancer cell-dendritic cell(DC) fusion vaccines and to provide theoretical data for biological therapy of gastric cancer based on anti-tumor fusion vaccines. Methods:(1)Peripheral blood mononuclear cells from gastric cancer patients were separated and co-cultured with granulocyte macrophage colony stimulating factors, interleukin-4 and tumor necrosis factor-α to generate mature dendritic cells. (2)The dendritic cells and SGC7901 cells were fusioned by use of polyethylene glycol, and the pure fusion cells were screened out and cultured with HAT and HT selective culture systems. (3)The ability of fusion cells to activate the cytotoxic T lymphocytes was investigated by using MLA method. Results:(1)Mature dendritic cells were gained from gastric cancer patients′ peripheral blood mononuclear cells by co-culturing with granulocyte-macrophage colony stimulating factors, interleukin-4 and tumor necrosis factor-α. (2)Dendritic cells and SGC7901 cells could be fusioned by PEG and could get pure fusion cells by culture with HAT/HT selective culture systems. (3)The fusion vaccines could strongly activate cytotoxic T lymphocytes, and there were significent differences between the fusion vaccine group and the control group. (P<0.01). Conclusions:Gastric cancer cell-dendritic cell fusion vaccine has stronger ability to stimulate the proliferation of T lymphocytes than that of parent dendritic cells, and may be a basis for induction of stronger anti-tunmor biological effects.