Abstract:Objective:To study the significance of polo like kinase1(plk1) mRNA and protein expression in gastric cancer tissues and the relationship between the plk1mRNA and protein expression level in gastric cancer tissues and the clinicopathological parameters. Methods:Plk1mRNA and protein level were respectively measured by real-time PCR and Western blot in fresh tumor tissues and the normal gistric mucosa from 60 patients with gastric cancer. Results:plk1mRNA and protein expression level in gastric cancer tissues was significantly higher than that in normal gastric tissues(P<0.05, P<0.01). plk1mRNA expression level in gastric cancer tissues was closely related to degree of tumor differentiation and depth of invasion(P<0.05), and plk1 protein level was closely related to tumor differentiation (P<0.05). Conclusions:The overexpression of plk1 may promote carcinogenesis and the development of gastric cancer, and plk1 can be a novel marker for determing certain biological behaviours of gastric cancer.