Abstract:Objective:To establish a simple and stable model of orthotopic small intestinal transplantation (OIT) in rats. Methods:The harvested en bloc segmental intestine consisted of donor abdominal aorta with superior mesenteric artery and portal vein. After in situ infusion with lactated Ringer′s solution , the graft was stored in 4℃ lactated Ringer′s solution. The segmental intestine transplantation was performed by end-to-side anastomosis of donor abdominal aorta to recipient abdominal aorta . The donor portal vein was anastomosed to the recipient left renal vein by "cuff anastomosis". The graft was anastomosed orthotopically by continuous sutures. Results:Sixteen intestine transplantations were, performed the average time for the arterial and venous anastomosis was 25±5 min and 4±1 min,respectively. Among the 16 recipient rats, 13 survived more than 5 days. The average survival time was 10.35±2.84 days; the longest survival time was 21 days. Conclusions:Graft harvesting, the technique of vascular and intestinal anastomosis and the maintenance of adequate blood volume are the key points for success of the operation. This successfully established model can serve as an excellent animal model for basic research of small intestinal trasplantation.