Abstract:Objective:To establish a novel liver and lung metastatic tumor model in nude mice by splenic inoculation of hilar cholangiocarcinoma cell line. Methods:Human hilar cholangiocarcinoma cell line(FPH-0201 cells) was established and cultured with a fresh human hilar cholangiocarcinoma specimen. The spleen of 7 nude mice was injected with 0.1ml of hilar cholangiocarcinoma cell suspension (1.5×107 cell) .The tumor appearing time, tumor local and metastatic formation rate, histological,morphological and ultrastructure of metastatic tumor cell were observed. The sub-cell line was obtained by cutture of metastatic specimen, and sub-cell line cells were again injected into the lower pole of the spleen and metastatic tumor formation rate was observed again. Results:The overall local tumor formation rate in spleen was 100%, and metastatic tumor formation rate was 14.3%. The sub-cell line cells that were re-inoculated in mice spleen, had a metastatic tumor formation rate of 100%. Morphological observation of metastatic tumor showed the common features of cholangiocarcinoma. Chromosome number showed a variation of 18~44. Conclusions:The hilar cholagiocarcinoma metastatic tumor model that was established in this laboratomy had characteristics compatible with malignant tumor and had all the biological characteristics of human hilar cholagiocarcinoma.