Abstract:Objective:To study the changes of intrahepatic shunt flow(IHSF) and functional hepatic blood flow(FHBF) after liver ischemia reperfusion in rats. Methods:12 healthy male SD rats were anaesthetized and heparinised.The right carotid artery,jugular vein and two distal ileocolic veins were cannulated for blood transfusion,volume compensation,drug infusion,and blood sampling respectively. Rats were randomly divided into 2 groups. Group 1 was the sham operation control group. Group 2 rats was ischmic/reperfusion(I/R) group, subjecting to 45min liver lobar ischemia and 60min reperfusion.Both groups received infusion of D-sorbitol(10mmol/l,0.2mL/min) via portal venous,and blood samples(1mL) were drawn simultaneously from the carotid,portal venous,and hepatic venous catheters.Portal venous flow(PVF) and hepatic arterial flow(HAF) were measured via flow probes.Hepatic sorbitol uptake rate, FHBF and IHSF were calculated. Results:There were no significant differences between the two groups in PVF,HAF and total hepatic blood flow(THBF). Compared to control group, hepatic sorbitol uptake rate as well as FHBF were decreased and IHSF was increased in I/R group(P<0.01). Conclusions:After liver ischemia reperfusion in rats, intrahepatic portal systemic shunts were opened and FHBF was reduced.