Abstract:Objective
To study the expression of BRMS1mRNA in human breast cancer tissues and their significance.
Methods The expression of BRMS1mRNA was detected by reverse transcriptionpolymerase chain reaction(RTPCR) in 71 breast cancer tissues and adjacent breast tissues,12 patients with benign breast tumors and 12 patients with normal breast tissue,and semiquantitative analysis of band densities was also performed.
Results The expression of BRMS1mRNA in 71 patients with breast cancer and adjacent breast tissue was 0.378±0.046 and 0.918±0.044, respectively; the expression of BRMS1mRNA in 12 patients with benign breast tumors and 12 patients with normal breast tissue was 0.908±0.047 and 0.934±0.028 respectively. BRMS1mRNA expression was significantly lower in breast cancer tissue compared to adjacent breast tissue,benign breast tumors and normal breast tissue (P<0.01) .The expression of BRMS1mRNA in breast cancer was reduced and had no relation with patient age,tumor size,and ER,PR (P>0.05), but was related to axillary lymph node metastasis and clinical stage(P<0.05). Conclusions The expression of BRMS1mRNA in patients with breast cancer was correlated with axillary lymph node metastasis and clinical stage, and the reduction of BRMS1mRNA expression could be an index for metastasis and prognosis of patients with breast cancer.