Abstract:Objective:To investigate the inhibitory effect of appling slow-releasing triptolide on adventitia on the intima hyperplasia of autologous vein graft.
Methods :In 24 male New Zealand rabbits, external jugular vein to common carotid artery models were established, and then were divided into 3 equal groups at random: blank-control group, receiving no management on adventitia of the vein graft; F-127 control group, receiving local application of 0.5 mL of 20 % F-127 on adventitia of the vein graft; experiment group, receiving local application of 0.5 mL of 20 % F-127 containing triptolide 300μg. Vein graft specimens were harvested at 2 weeks after the operation. Histomorphologic methods were used to detect the degree of intima hyperplasia of the specimens. The expression of bcl-2 and Fas of the specimens was detected by immunohistochemistry. The apoptosis vascular smooth muscle cells (VSMC) were detected by TUNEL.
Results:Two weeks after vein grafting, compared to blank-control group and F-127 control group, intima hyperplasia of experiment group ［intima thickness （29.9±7.6）μm, I/M 0.56±0.08］ was markedly inhibited (P<0.05). Expression of bcl-2［(18.2±8.4)%］ was reduced significantly, however, expression of Fas(21.4±8.9）% increased markedly, and the apoptotic cells ［(28.4±7.6)%］ also increased markedly (P<0.05).
Conclusions:Applied slow-releasing triptolide by F-127 pluronic on the vein graft adventitia can effectively inhibit intima hyperplasia of vein graft by a mechanism of enhancement of VSMC apoptosis.