Abstract:Objective:To study the influence of pcDNA3.1-RhoC on the expression of endogenous angiogenic factors in HCC cells.
Methods :The reconstructed plasmid pcDNA3.1-RhoC was transfected into HepG2 cells, and expression of VEGF and bFGF was detected with the RT-PCR and immunohistochemical stain. HepG2 cells transfected with pcDNA3.1-RhoC or pcDNA3.1 were implanted into nude mice to observe the tumor occurrence rate.
Results:HepG2 cells transfected with pcDNA3.1/RhoC showed higher expression of RhoC . The expression of RhoC enhanced the expression of VEGF and bFGF(P＜0.01).The tumor occurrence rate in nude mice implanted with HepG2 cells transfected with pcDNA3.1-RhoC(20 %) was higher than those implanted with HepG2 cells transfected with pcDNA3.1 (0 %).
Conclusions:The expression of RhoC efficiently enhances the expression of VEGF and bFGF;RhoC can promote the expression of angiogenic factors in HCC cell, and it may be one of the mechanisms of RhoC for enhancement of invasion and metastasis of HCC.