Abstract:Objective:To explore the role of 1, 4, 5-trisphosphate inositol ( IP3) and bax gene expression in apoptosis of HepG2 cells induced by Quercetin.
Methods :HepG2 cells were cultured for 72h as control. HepG2 cells were treated with different concentrations including 20,40.60,80μmol/L Quercetin for 72h, and treated with 60μmol/ L Quercetin for 12h, 24h, 48h and 72h. IP3, bax mRNA, bax protein and apoptosis rate were assayed by IP3-［3H］ Birtrak assay,RT-PCR, Western blotting and flow cytometry, respectively.
Results:In HepG2 cells incubated with each of the concentrations of Quercetin for 72 h，IP3continent was lower than that of control(P<0.01)，bax mRNA expression and bax protein expression was higher than that of control，and the apoptosis rate was higher than that of control; in HepG2 cells incubated with Quercetin for 6 h, 12 h, 24 h, 48 h, 72 h, IP3content was lower than that of control(P<0.01);bax mRNA and bax protein expression in groups incubated with 60 μmol/L Quercetin for 12h was higher than that of control， and the apoptosis rate in groups incubated with 60μmol/ L Quercetin for 24h, 48h and 72h was significantly higher than that in control (P<0.01).
Conclusions:Quercetin induces apoptosis of HepG2 cells by reducing IP3production and up-regulating bax gene expression.