Abstract:Objective:To study the effect of magnetic nanoparticles on human cholangiocarcinoma xenograft in nude mice, and compared with otherchemotherapy drugs
Methods:We established human cholangiocarcinoma xenograft in nude mice with QBC939 cell line.The nude mice were devided into 4 groups randomly.Saline,5-FU, Gemcitabine and magnetic nanoparticles were given to nude mice through tail vein on 20d after implanting QBC939 cell line. Calculations were done at different time after treatment in order to compare tumor volume,inhibition ratio of tumor and tumor growth curve of each group. The nude mice were killed on 35d after treatment to harvest tissue for electron microscopic examination to observe ultra-structural changes. Results:The tumor volume of control, 5-FU, magnetic nanoparticles and Gemcitabine groups was (2256.1±267.1) mm3, (2096.5±237.9)mm3,(1392.2±189)mm3, and (1534.9±115 )mm3 respectively.The last two groups have significant difference compared to the first two groups（P<0.05）. The ratio of inhibition of tumor and apotosis in the chemotherapy treated groups were 7.4%,39.6%, and 33.0% respectively. Conclusions:The effect of targeting drug magnetic nanoparticles was better than equivalent dosage of 5-FU, but as the same of Gemcitabine.