Abstract:Objective:To investigate the effect of Bcl-2 ASODN on VP-16 induced growth inhibition and apoptosis in human bile duct carcinoma cell line QBC939.
Methods : Human cholangiocarcinoma cell line QBC939 was conventionally cultured. Specific target selective Bcl-2 ASODN，designed and artificially synthesized，was transfected to the cell line;MTT assay and flow cytometry (FCM) were used to study cell proliferation and apoptosis in cell line QBC939.
Results: MTT assay showed cell survival rate in ASODN group was significantly lower than that in NSODN group(P＜0.05).The survival rate in ASODN+VP-16 group was significantly lower than that in ASODN group and VP-16 group(P＜0.05). FCM analysis showed that apoptosis rates in ASODN group, VP-16 group and ASODN+VP-16 group were significantly higher than those in control groups(P＜0.05). The apoptosis rate in ASODN +VP-16 group was significantly higher than that in ASODN group and VP-16 group (P＜0.05).
Conclusions:Bcl-2 ASODN can enhance the effect of VP16-induced growth inhibition and apoptosis in QBC939 cell line.