Abstract:Objective:To explore an improved technique of mouse ventral heterotopic heart transplantation and construct an animal model for the study of transplantation immunity.Methods:The donor heart aorta and the recipient ventral aorta, the donor pulmonary artery and the recipient inferior caval vein, were anastomosed by using the end-to-side suture technique respectively. At the same time, important anatomical structures of transplantation-related sites were measured.Results:The diameter of recipient inferior caval vein and ventral aorta was (1.9±0.2)mm and (0.9±0.1)mm respectively. The average length of ventral blood vessels which can be used was (6.0±1.0)mm. The model construction success rate was 92%. The average time of donor operation and recipient operation was (8.0±1.0)min and (40.2±3.0)min respectively. The time of artery anastomosis and vena cava anastomosis was (9.3±1.2)min and (7.8±1.7)min respectively. Conclusions:The animal model is stable and can be used for the study of transplantation immunity.