Abstract:Objective：To study expression of CD147 in rabbit VX2 tumor tissues after transarterial embolization with hydroxyapatite nanoparticles loaded with lipiodol.
Methods ：Thirty rabbits implanted with tumor tissue of cell line VX2 were divided into three groups: control group, lipiodol group, and hydroxyapatite nanoparticles loaded with lipiodol group. The transarterial embolization was performed superselectively via gastroduodenal artery of rabbits, and each rabbit in control group was infused with 1ml normal saline, that in lipiodol group was infused with 0.3ml lipiodol per kg, and o.3ml hydroxyapatite nanoparticles loaded with lipiodol per kg for that in the last group.Results of treatment were detected by using CT on day 3 after operation. After two weeks, three-step Immunohistochemical technique(S-P) and Western blot technique were used to investigate the expression of CD147 in tumor tissues of the 3 groaps. Results：Immunohistochemical results showed that CD147 was expressed on membrane of tumor cells in all three groups. The positive ratios of CD147 in three groups were （31.33±5.88）%,（75.63±4.44）%, and （80.03±5.59）% respectively, while the expression of CD147 in tumor tissues in control group was significantly different from others（P<0.01）, but for the other two groups, there was no difference between them（P＞0.05）. Protein half-quantitation detecting of CD147 with Western blot technique displayed that expression of CD147 in tumor tissues increased after embolization in last two groups as compared to that of control group（P<0.05）.Conclusions：There was an increasing tendency expression of CD147 in tumor tissues after transarterial embolization with hydroxyapatite nanoparticles loaded with lipiodol or only with lipiodol, and it may be one of the important factors for the high recurrence rate of hepatocellular carcinoma after embolization treatment.